Get guaranteed-accurate VH/VL sequences in 1 week
Biointron sequences the full variable regions of your hybridoma fast and with certainty. Using 5′ RACE, we clone and sequence the heavy and light chains from hybridoma cell lysates to preserve complete, expression-ready coding regions for downstream use. Standard input is two vials of lysate (~1×10⁶ cells each); if cells aren’t viable, we can sequence from extracted RNA (and provide RNA extraction if needed). You’ll receive a final sequence report with CDR annotations, raw sequence data, and sequencing plasmids containing the antibody coding sequence. Choose VH, VL, or full V-region, and, if desired, move straight into recombinant antibody production and purification.
Our antibody discovery services also include:
