With the growing demand for research-based antibodies, Biointron has launched a high-throughput recombinant antibody production service to support antibody drug screening. High-throughput recombinant antibody production service uses CHO cells or HEK293 cells as host cells to produce high-throughput and high-quality recombinant antibodies through a standardized and rapid antibody expression technology platform.
Biointron's Exclusive High Throughput Gene to Antibody Service
Delivery in 2 weeks
*Fastest turnaround time
(from gene synthesis to purified antibody)
|Customer provides||Scale and format||Premium service details||Timeline||Steps||Deliverables|
|VH/VL sequence subtype selection that you need||0.1 mg to 10 grams, any IgG species or subtype||Cloning|
Buffer exchange (PBS)
Purity >98% (SDS-PAGE)
Monomer purity >95% (HPLC)
Endotoxin < 1EU/mg
|2-3 weeks||Codons optimization and sequence design ||Purified antibody QC report|
|Hybridoma cells||Gene synthesis and expression vector construction|
|Biologic carrier library|
|Transient production and affinity purification|
|Quality control: SDS-PAGE, AEC-HPLC, LAL endotoxin test|
Competitiveness of Recombinant Antibody
Compared with traditional monoclonal antibody and polyclonal antibody, recombinant antibody has several advantages as follows.
Higher consistency and repeatability
Antibody production quality is controlled by a unique set of genes, and batch-to-batch variation in antibodies is very small. Some problems in the preparation of hybridoma cells, such as gene loss, gene mutation and cell line drift, can be avoided.
Stronger sensitivity and specificity
With recombinant technology, it is easier to improve antibody specificity and sensitivity through antibody engineering. The selection process for the desired clones occurs at the stage of hybridoma cells and recombinant clones, which allowed us to select the optimal antibody quality.
High throughput, large scale and fast speed
Compared with the conventional monoclonal technique, antibody expression can be performed on a single clone of 100+ at a shorter time in any scale. We can deliver antibodies to customers within two weeks.
High throughput production with animal-free culture
Once antibody producing gene is isolated, animal-free in vitro production can be achieved.
SDS-PAGE and SEC-HPLC Detection